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Part:BBa_K784021:Design

Designed by: Hila Korach Rechtman   Group: iGEM12_Technion   (2012-09-17)

Phage Lambda fragment 7 - replication and lytic regulation


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 689
    Illegal EcoRI site found at 6493
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 689
    Illegal EcoRI site found at 6493
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 689
    Illegal EcoRI site found at 6493
    Illegal BglII site found at 275
    Illegal BglII site found at 335
    Illegal BamHI site found at 3253
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 689
    Illegal EcoRI site found at 6493
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 689
    Illegal EcoRI site found at 6493
    Illegal AgeI site found at 1973
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4236


Design Notes

This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation.
The main goal with the genome deviation to eight fragments was to avoid putting regulatory elements with their regulated sequences. By doing so, we can prevent the expression of harmful and lytic associated proteins to the host bacteria. Moreover, due to the phage lambda genome organization we could divide the phages genome while each part contains several proteins which are in functional correlation with each other.

Source

http://www.ncbi.nlm.nih.gov/nuccore/NC_001416.1

The physical DNA was obtained from NEB - http://www.neb.com/nebecomm/products/productn3011.asp


References